A team of scientists used gene editing to eliminate HIV DNA in live mice

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A team of scientists have snipped away HIV DNA from the genome of live mice using a CRISPR system, and the rodents lived to tell (kinda) the tale. It’s still much too early to call the method a possible cure, but the fact that it worked on a living animal opens up a lot of possibilities.
Will it work on other diseases, like cancer? Maybe, but that’s something scientists have to look into. These researchers, headed by neurovirologist Kamel Khalili, have been focusing on the use of the gene-editing technique to eliminate HIV for years. They successfully excised HIV DNA in live mice last year, but this round is a lot more thorough.


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The team used three different types of mice for their most recent experiments. First type is infected with latent HIV lurking in its cells, the same type they used for their trials in 2016, while the second one has actively replicating HIV in its system. The last type is grafted with human immune cells, including T cells that have latent HIV in them. HIV can actually hide in the immune cells that are supposed to kill them, which is one of the reasons why it’s hard to find a cure for the condition. While anti-retroviral therapies allow HIV patients to live a normal life, they’re far from perfect.
“The current anti-retroviral therapy for HIV is very successful in suppressing replication of the virus,” Khalili explained. “But that does not eliminate the copies of the virus that have been integrated into the gene, so any time the patient doesn’t take their medication, the virus can rebound.”
To introduce their CRISPR system into mice without being attacked by its immune system, the researchers attached it to adeno-associated virus that doesn’t typically trigger a response in the body like other viruses do. The system eliminated the rodents’ HIV as the AA virus replicated. While successful, the team still has to make sure their system only snipped away the HIV and none of the good DNA. That’s why they’re planning to repeat the experiments on primates, whose DNA is closer to humans. Ultimately, they hope to follow in the footsteps of the Chinese oncologists from Sichuan University who already conducted CRISPR human trials.



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